Here's a paragraph about the molds that may help to infer the reason of the advantages during observation of mold colonies: 'Because the structural components of molds are very delicate, even simple handling with an inoculating loop may result in mechanical disruption of their components.The followi ng culture technique es used to to avoid this disruption. After culturing, molds spores are deposited in the surface of the agar and incubated in a moist chamber at room temperature. Direct microscopic observation is then possible without fear of disruption or damage to the anatomical components'. It may be more beneficial in this aspect: allowing a full and healthy growth of the mold, that is, with its complete structure, then to be able to proceed to look through the microscope maybe it form, type of spore,sporangia or mycelium. In short, ensure that the mold to grow properly. Maybe it's not what you exactly were looking for but it could help you!

The dilution factor chosen for the series of calibration standards is achievable by using serial dilution. The progression of calibration standard concentration is always a geometric series. Consider the example of making the first standard at 1/3 the concentration of the known, the next calibrant would be 1/9th the concentration of the known and the following two calibrants formed are 1/27th and 1/81st. Plates are incubated anaerobically at 37oC for 3–4 days. Serial dilutions are used for determination of cfu as described above for lactobacilli. However, in the presence of accompanying micro flora, AMC agar (Arroyo et al. 2001) with the aid of the primer pairs which may be genus-specific, as is the case with bifidobacteria.

Second Year of Microbiology bachelor's degree (University Of Puerto Rico in Arecibo) Paragraph taken of Capuccino/Sherman Microbiology. A laboratory Manual.Eight Edition. The following are some advantages of an agar plate verses a slant tube: 1. Surface area- An agar plate has a much larger surface area: a.

Easier to isolate individual colonies using the streak-plate method. Evaluate the colony shape, margin and elevation. Can grow a larger number of ce lls. Growth- An agar plate allows you to quantify the number of colonies on an agar plate, provided it is within the 30-300 range.

Whereas the slant tube cannot quantify growth but only describes growth as none, slight, moderate, or large.

According to the video on the proper aseptic techniques for use in a laboratory, one reason that aseptic technique is necessary is to protect oneself from contact with biohazards that exist within a lab setting. Additionally, it serves the purpose to protect samples from being contaminated. Aseptic technique is also extremely important for safeguarding others in the lab. Since bacteria are everywhere, including on all of the lab equipment, aseptic techniques allow us to avoid the spread of infection in the above ways. The concentration of bleach that is used to disinfect your work area is a 10% concentration of bleach.

The bleach concentration serves as a disinfectant, which is an agent that is intended to kill or remove microorganisms, but it does not kill bacteria spores. It is not a method of sterilization because sterilization is the process used to remove all life forms, including bacteria spores. It is important to disinfect your work area before and after working. Do not trust that the person before you took the time to follow disinfection procedure, so always be sure to be consistent just in case. The video demonstrates the need for the step of passing the mouth of the tube through the flame. This is in order to keep the tube free of contamination.

The increased temperature at the mouth of the tube works to create a confection-oven-like current, which pushes the air out of the tube and eliminates the potential for airborne contaminants to get into the mouth of the tube. The presence of the Bunsen Burner in the lab area also serves to decrease the amount of airborne contamination, but this process ensures that anything that may still be present in the air does not enter the tube. The correct way to successfully perform this procedure is to pass the tube over the flame at a 45-degree angle. A pure culture is a culture that only has one type of microorganism growing in it. As a result, if you only see one type of microorganism growing in the culture, then you know that you have used the aseptic technique correctly and have achieves a pure culture.

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